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Oct-4A (red) & E-Cadherin (green) in iPS2 human induced pluripotent stem cells. See Details

CD90/Thy1 & CD45 in human mesenchymal stem cells. See Details

SSEA-1 (red) & SSEA-4 (green) in live iPS2 human induced pluripotent stem cells. See Details

Recent Publications Citing R&D Systems Featured Products for Definitive Endoderm Differentiation

1. Ratcliffe, E. et al. (2013) Application of response surface methodology to maximize the productivity of scalable automated human embryonic stem cell manufacture.
   Regen. Med. 8:39.

   PE-conjugated Mouse Anti-Human Oct-4A Monoclonal Antibody (Catalog # IC6344P)
   PE-conjugated Mouse Anti-Human/Mouse SSEA-1 Monoclonal Antibody (Catalog # FAB2155P)
   PE-conjugated Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # FAB1435P)
   PE-conjugated Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # FAB1448P)
   Sample: Human mesenchymal stem cells
   Application: Flow cytometry



2. Takemitsu, H. et al. (2012) Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
   BMC Vet. Res. 8:150.

   PE-conjugated Mouse Anti-canine CD34 Monoclonal Antibody (Catalog # FAB3346P)
   Mouse Anti-Human/Mouse SSEA-1 Monoclonal Antibody (Catalog # MAB2155)
   Mouse Anti-Human/Mouse SSEA-3 Monoclonal Antibody (Catalog # MAB1434)
   Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # MAB1435)
   Mouse Anti-Human TRA-1-60 (R) Monoclonal Antibody (Catalog # MAB4770)
   Sample: Canine bone marrow or adipose-derived mesenchymal stem cells
   Application: Flow cytometry



3. Azarin, SM. et al. (2012) Effects of 3D microwell culture on growth kinetics and metabolism of human embryonic stem cells.
   Biotech. & App. Biochem 59:88.

   Goat Anti-Human E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648)
   Sample: Human embryonic stem cells
   Application: Immunocytochemistry



4. Buchheiser, A. et al. (2012) Oxygen tension modifies the ‘stemness’ of human cord blood-derived stem cells.
   Cytotherapy 14:967.

   Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430)
   Mouse Anti-Human CD90/Thy1 Monoclonal Antibody (Catalog # MAB2067)
   Fluorescein-conjugated Mouse Anti-Human MCAM/CD146 Monoclonal Antibody (Catalog # FAB932F)
   Sample: Human cord blood-derived stem cells
   Application: Flow cytometry



5. Giuliani, M. et al. (2011) Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
   PLoS One 6:e19988.

   PE-conjugated Mouse Anti-Human CD90/Thy1 Monoclonal Antibody (Catalog # FAB2067P)
   PE-conjugated Mouse Anti-Human Endoglin/CD105 Monoclonal Antibody (Catalog # FAB10971P)
   PE-conjugated Mouse Anti-Human ALCAM Monoclonal Antibody (Catalog # FAB6561P)
   Sample: Human mesenchymal stem cells
   Application: Flow cytometry

   Human/Mouse/Rat StemXVivo™ Chondrogenic Base Media (Catalog # CCM005)
   Human/Mouse StemXVivo Chondrogenic Supplement (Catalog # CCM006)
   Human/Mouse/Rat StemXVivo Osteogenic/Adipogenic Base Media (Catalog # CCM007)
   Human StemXVivo Osteogenic Supplement (Catalog # CCM008)
   Human StemXVivo Adipogenic Supplement (Catalog # CCM011)
   Sample: Human mesenchymal stem cells
   Application: Differentiation
   
   Human IL-15 Quantikine^® ELISA Kit (Catalog # D1500)
   Sample: Human mixed-lymphocyte reaction cell culture supernates
   Application: ELISA

   PE-conjugated Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # FAB3791P)
   PE-conjugated Mouse Anti-Human CD8 alpha Monoclonal Antibody (Catalog # FAB1509P)
   PE-conjugated Mouse Anti-Human IL-2 R alpha Monoclonal Antibody (Catalog # FAB1020P)
   PE-conjugated Mouse Anti-Human CD28 Monoclonal Antibody (Catalog # FAB342P)
   PE-conjugated Mouse Anti-Human CD69 Monoclonal Antibody (Catalog # FAB23591P)
   Sample: Human T cells
   Application: Flow cytometry

Figure Legends

Oct-4A (red) & E-Cadherin (green) in iPS2 human induced pluripotent stem cells. Oct-4A and E-Cadherin were detected in iPS2 human induced pluripotent stem cells using a Mouse Anti-Human Oct-4A Monoclonal Antibody (Catalog # MAB17591) and a Goat Anti-Human E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648). The cells were stained for Oct-4A using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and stained for E-Cadherin using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Specific staining of Oct-4A in the nucleus and E-Cadherin at the plasma membrane suggests this population of cells is pluripotent. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

CD90/Thy1 & CD45 in human mesenchymal stem cells. Human mesenchymal stem cells (MSC) were stained with a PE-conjugated Mouse Anti-Human CD90/Thy1 Monoclonal Antibody (Catalog # FAB1430P) and an APC-conjugated Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # FAB2067A). Gate based on control antibodies. The data show high levels of CD90/Thy1 and low expression of CD45, a phenotype that is consistent with a multipotent MSC starting population.

SSEA-1 (red) & SSEA-4 (green) in live iPS2 human induced pluripotent stem cells. iPS2 human induced pluripotent stem cells were grown on irradiated mouse embryonic fibroblasts (Catalog # PSC001) and stained using the NL493-conjugated SSEA-4 (green) and NL557-conjugated SSEA-1 (red) antibodies included in the Human Pluripotent Stem Cell Live Cell Imaging Kit (Catalog # SC023). The cells were counterstained with Hoechst 33342 (blue). The colonies are positive for the stem cell marker SSEA-4 and are negative for SSEA-1, suggesting that these colonies primarily contain undifferentiated human stem cells. This kit also contains a NL557-conjugated Mouse Anti-Human TRA-1-60(R) Monoclonal Antibody.